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ThinkMind // ICQNM 2013, The Seventh International Conference on Quantum, Nano and Micro Technologies // View article icqnm_2013_5_10_80050


Design of Biosensors with Extended Linear Response and Binary-Type Sigmoid Output Using Multiple Enzymes

Authors:
Oleksandr Zavalov
Sergii Domanskyi
Vladimir Privman
Aleksandr Simonian

Keywords: enzyme biosensor; linear response; differential sensitivity; intensity filter

Abstract:
We review results on theoretical modeling of biochemical systems in support of the studies of several types of biosensors and systems for "biocomputing" information processing. The developed theoretical techniques have been utilized to investigate performance and optimize a novel approach to biosensors with extended linear response for lysine detection and also to study a "binary" response of a metabolic "branch point effect" system for detection of glucose. We study the experimental data for the flow-injection amperometric biosensor based on the action of Lysine-2-monooxygenase and L-Lysine-alpha-Oxidase. Lysine is a homotropic substrate for both enzymes. Parameter values are identified for an extended linear range of response. For a "branch point effect" system, we demonstrate that an "intensity filter" mechanism can yield a sigmoid response useful in biochemical signal processing and enzyme-based biosensing applications.

Pages: 54 to 59

Copyright: Copyright (c) IARIA, 2013

Publication date: August 25, 2013

Published in: conference

ISSN: 2308-3530

ISBN: 978-1-61208-303-2

Location: Barcelona, Spain

Dates: from August 25, 2013 to August 31, 2013

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