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ThinkMind // SENSORDEVICES 2011, The Second International Conference on Sensor Device Technologies and Applications // View article sensordevices_2011_9_20_20127


Cell chip to analyze cell lines and cell cycle stages based on electrochemical method

Authors:
Md. Abdul Kafi
Tae-Hyung Kim
Jeong-Woo Choi

Keywords: Cyclic voltammetry; Differential pulse voltammetry; Cell cycle progression; Cell chip

Abstract:
Cell chip was fabricated to analyze different cell lines and cell cycle-stages based on the electrochemical tools. Cell-based biochips are becoming promising tools in the various kinds of biotechnology field such as early diagnosis, cell therapy and drug screening. In this study, cells were immobilized on collagen coated gold surface and subjected to voltammetric methods including cyclic voltammetry and differential pulse voltammetry. The distinct redox phenomena were detected at the cell-electrode interface which varies with different kinds of cell lines. Moreover, the redox peaks were found to be different from same cell line but in different cell cycle stages. A typical sigmoid curve was achieved between the electrochemical signals and cell concentrations that proved the reproducibility of electrochemical signals. Hence, our developed cell chip based on the electrochemical tool can be applied for the differentiation of metastatic cancer cells from unknown origin, as well as for the assessment of cell cycle-dependent effects of drugs or toxins.

Pages: 147 to 150

Copyright: Copyright (c) IARIA, 2011

Publication date: August 21, 2011

Published in: conference

ISSN: 2308-3514

ISBN: 978-1-61208-145-8

Location: Nice/Saint Laurent du Var, France

Dates: from August 21, 2011 to August 27, 2011

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